Genotyping and primer details


Genotyping and primer details for 575C05 - At5g19070
Line ID 575C05
Locus At5g19070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:6376552 of line 575C05 Chr5:6376552
   
Insertion allele amplicon 8474 - 52by
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 52by: 5'-CAATGAGCTACACTAACGGTGGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:6376065
   
wt amplicon (over InsPos) 19dd - 52by
wt amplicon start Chr5:6376065
wt amplicon end Chr5:6376865
wt amplicon size 801 bp
Locus specific primer 52by: 5'-CAATGAGCTACACTAACGGTGGTA-3'
2nd locus specific primer 19dd: 5'-ACGAAGAAAGCAAGTATGGACCTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11