Genotyping and primer details


Genotyping and primer details for 602A05 - At4g13730
Line ID 602A05
Locus At4g13730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:7971041 of line 602A05 Chr4:7971041
   
Insertion allele amplicon 8474 - 05kx
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05kx: 5'-ATAACACCCTTAGCTACTGGCAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:7971488
   
wt amplicon (over InsPos) 06li - 05kx
wt amplicon start Chr4:7970641
wt amplicon end Chr4:7971488
wt amplicon size 848 bp
Locus specific primer 05kx: 5'-ATAACACCCTTAGCTACTGGCAAC-3'
2nd locus specific primer 06li: 5'-TGCTCTATGCATTTTGATTGATTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11