Genotyping and primer details


Genotyping and primer details for 710E12 - At3g61113
Line ID 710E12
Locus At3g61113 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22615146 of line 710E12 Chr3:22615146
   
Insertion allele amplicon 8474 - 28us
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 28us: 5'-GTATCGAGCTGACCACTAAGTTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22614637
   
wt amplicon (over InsPos) 42vc - 28us
wt amplicon start Chr3:22614637
wt amplicon end Chr3:22615604
wt amplicon size 968 bp
Locus specific primer 28us: 5'-GTATCGAGCTGACCACTAAGTTCC-3'
2nd locus specific primer 42vc: 5'-TTGGGTATTTTTAAGAAGGGCTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11