Genotyping and primer details


Genotyping and primer details for 737G08 - At5g19080
Line ID 737G08
Locus At5g19080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:6379363 of line 737G08 Chr5:6379363
   
Insertion allele amplicon 8474 - 05me
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05me: 5'-ATTATCTTGTTTCCTTCGTCTTCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:6378875
   
Insertion allele amplicon 8474 - 58lm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 58lm: 5'-TATCTTGTTTCCTTCGTCTTCGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:6378877
   
wt amplicon (over InsPos) 10vu - 58lm
wt amplicon start Chr5:6378877
wt amplicon end Chr5:6379886
wt amplicon size 1010 bp
Locus specific primer 58lm: 5'-TATCTTGTTTCCTTCGTCTTCGAT-3'
2nd locus specific primer 10vu: 5'-TAGGTTCAGTCAAGCAAATGACAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11