Genotyping and primer details


Genotyping and primer details for 759B02 - At1g75110
Line ID 759B02
Locus At1g75110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28195041 of line 759B02 Chr1:28195041
   
Insertion allele amplicon 8474 - 12kz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 12kz: 5'-CTTAGAGAGTGTATCTGCCACACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28194636
   
wt amplicon (over InsPos) 15ls - 12kz
wt amplicon start Chr1:28194636
wt amplicon end Chr1:28195533
wt amplicon size 898 bp
Locus specific primer 12kz: 5'-CTTAGAGAGTGTATCTGCCACACG-3'
2nd locus specific primer 15ls: 5'-AATTGCATGTCTTGAACATTTGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11