Genotyping and primer details


Genotyping and primer details for 794C09 - At3g50920
Line ID 794C09
Locus At3g50920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18922603 of line 794C09 Chr3:18922603
   
Insertion allele amplicon 8474 - 17um
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17um: 5'-AATCTTGATTTTAACAAACGGGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18922300
   
wt amplicon (over InsPos) 71vo - 17um
wt amplicon start Chr3:18922300
wt amplicon end Chr3:18922941
wt amplicon size 642 bp
Locus specific primer 17um: 5'-AATCTTGATTTTAACAAACGGGCT-3'
2nd locus specific primer 71vo: 5'-TTCTCATGCTCAATCCATTTCTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11