Genotyping and primer details


Genotyping and primer details for 818D11 - At2g02780
Line ID 818D11
Locus At2g02780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:782184 of line 818D11 Chr2:782184
   
Insertion allele amplicon 8474 - BB12
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BB12: 5'-TGTTTTTCAACTCCTTTTGCTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:781789
   
Insertion allele amplicon 8474 - EU76
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU76: 5'-ATTTCTAGGTTGGAGAAGAGAGGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:782512
   
wt amplicon (over InsPos) BB12 - EU76
wt amplicon start Chr2:781789
wt amplicon end Chr2:782512
wt amplicon size 724 bp
Locus specific primer EU76: 5'-ATTTCTAGGTTGGAGAAGAGAGGC-3'
2nd locus specific primer BB12: 5'-TGTTTTTCAACTCCTTTTGCTTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11