Genotyping and primer details


Genotyping and primer details for 835F07 - At1g49830
Line ID 835F07
Locus At1g49830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18445822 of line 835F07 Chr1:18445822
   
Insertion allele amplicon 8474 - 15gg
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15gg: 5'-CAAGACATAAACCATTGCTTCTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18445251
   
wt amplicon (over InsPos) 03hi - 15gg
wt amplicon start Chr1:18445251
wt amplicon end Chr1:18446213
wt amplicon size 963 bp
Locus specific primer 15gg: 5'-CAAGACATAAACCATTGCTTCTCA-3'
2nd locus specific primer 03hi: 5'-TCTTTGTTTTGAAGAAGAAAACCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11