Genotyping and primer details


Genotyping and primer details for 852A11 - At1g77450
Line ID 852A11
Locus At1g77450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29100228 of line 852A11 Chr1:29100228
   
Insertion allele amplicon 8474 - 47bk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 47bk: 5'-GGCATCTACGTAATTAAACGGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29100885
   
wt amplicon (over InsPos) 05cu - 47bk
wt amplicon start Chr1:29099794
wt amplicon end Chr1:29100885
wt amplicon size 1092 bp
Locus specific primer 47bk: 5'-GGCATCTACGTAATTAAACGGAAA-3'
2nd locus specific primer 05cu: 5'-GTCCCTCTCCATCTACTTCAAATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11