Genotyping and primer details


Genotyping and primer details for 860G07 - At5g62720
Line ID 860G07
Locus At5g62720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25193519 of line 860G07 Chr5:25193519
   
Insertion allele amplicon 8474 - 21nt
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 21nt: 5'-ACGAGTAATGACCATAAAAGCGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25194056
   
wt amplicon (over InsPos) 05or - 21nt
wt amplicon start Chr5:25192976
wt amplicon end Chr5:25194056
wt amplicon size 1081 bp
Locus specific primer 21nt: 5'-ACGAGTAATGACCATAAAAGCGAT-3'
2nd locus specific primer 05or: 5'-TTAGTCAAACCCTATGCTGACAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11