Genotyping and primer details


Genotyping and primer details for 892H04 - At3g19500
Line ID 892H04
Locus At3g19500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6760310 of line 892H04 Chr3:6760310
   
Insertion allele amplicon 8474 - NA33
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NA33: 5'-TGGTGGAGAAGACAGTTTAGGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6760753
   
wt amplicon (over InsPos) 11hs - NA33
wt amplicon start Chr3:6759906
wt amplicon end Chr3:6760753
wt amplicon size 848 bp
Locus specific primer NA33: 5'-TGGTGGAGAAGACAGTTTAGGTTT-3'
2nd locus specific primer 11hs: 5'-TCTTTTTGAAGTTCACATTGGATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11