Genotyping and primer details


Genotyping and primer details for 910B06 - At2g24290
Line ID 910B06
Locus At2g24290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10339799 of line 910B06 Chr2:10339799
   
Insertion allele amplicon 8474 - 82ns
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 82ns: 5'-TTGTTAGTGACTCTTGACCCACAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10340363
   
wt amplicon (over InsPos) 04om - 82ns
wt amplicon start Chr2:10339347
wt amplicon end Chr2:10340363
wt amplicon size 1017 bp
Locus specific primer 82ns: 5'-TTGTTAGTGACTCTTGACCCACAC-3'
2nd locus specific primer 04om: 5'-AACCAATGGGTGCAACTATATCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11