Genotyping and primer details


Genotyping and primer details for 936E04 - At1g73650
Line ID 936E04
Locus At1g73650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27688611 of line 936E04 Chr1:27688611
   
Insertion allele amplicon 8474 - 87ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 87ra: 5'-TTTTTATTTGCCTGTGTATTCGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27688037
   
wt amplicon (over InsPos) 49si - 87ra
wt amplicon start Chr1:27688037
wt amplicon end Chr1:27688965
wt amplicon size 929 bp
Locus specific primer 87ra: 5'-TTTTTATTTGCCTGTGTATTCGTG-3'
2nd locus specific primer 49si: 5'-ATCTTGTCATATTCGGACCACTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11