Genotyping and primer details


Genotyping and primer details for 968B05 - At3g01600
Line ID 968B05
Locus At3g01600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:230865 of line 968B05 Chr3:230865
   
Insertion allele amplicon 8474 - 73sm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 73sm: 5'-ATATGACTTCAGTTGCGGAGACTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:231302
   
wt amplicon (over InsPos) 12ti - 73sm
wt amplicon start Chr3:230528
wt amplicon end Chr3:231302
wt amplicon size 775 bp
Locus specific primer 73sm: 5'-ATATGACTTCAGTTGCGGAGACTT-3'
2nd locus specific primer 12ti: 5'-TTGTTTCCAAGATCACATATCAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11