General info on the pAC161 T-DNA
The figure below gives an overview of the T-DNA from pAC161. For the differences between pAC106 and pAC161 please check the download page.
Primer name | Sequence (5' to 3') |
---|---|
o2588
|
CGCCAGGGTTTTCCCAGTCACGACG
|
o3144/35St
|
GTGGATTGATGTGATATCTCC
|
o3269
|
GAAGGCGGGAAACGACAATCTG
|
o8409
|
ATATTGACCATCATACTCATTGC
|
o8418
|
GCAATGAGTATGATGGTCAATATG
|
o8474
|
ATAATAACGCTGCGGACATCTACATTTT
|
o8648
|
TATCTGGGAATGGCGAAATC
|
o8738
|
GGCTACACTGAATTGGTAGCTCA
|
o8760
|
GGGCTACACTGAATTGGTAGCTC
|
o8738
|
GGCTACACTGAATTGGTAGCTCA
|
o9525
|
CCACACGTGGATCGATCCGTCG
|
o10706
|
GAACCCTAATTCCCTTATCTGGG
|
Sul2
|
GTCGAACCTTCAAAAGCTGAAGT
|
Sul4
|
ATTTCACACAGGAAACAGCTATGA
|
Sul7
|
GACTACAGTCAGCCGTGCTTC
|
Sul9
|
GGTTTCCGAGATGGTGATTG
|
CR3S
|
TTGAGCATATAAGAAACCCTTAGT
|
The following primers are not specific to pAC161. They are used for the generation of GABI-Kat FSTs and work with all of the GABI-Kat vectors. Click here to get more information concerning the FST generation method.
Primer name | Sequence (5' to 3') |
---|---|
ADP2
|
GTAATAGCACTCAGTATAGGCGTGCAGCGGCCGGGCAGGT
|
ADP3
|
TAACCTGCCCAA
|
AP1m
|
CCATGGTAATAGCACTCAGTATAGGCG
|
AP2A
|
AGCGGCCGGGCAGGTTAGA
|
AP2G
|
AGCGGCCGGGCAGGTTAGG
|
AP2C
|
AGCGGCCGGGCAGGTTAGC
|
AP2T
|
AGCGGCCGGGCAGGTTAGT
|
Note 1: As mentioned above, the primers in the upper table are specific for pAC161 with the exception of CR3S!
Note 2: Please check FAQ 2 for lines that are NOT created with pAC161. For example, with pAC106 the combination o9525 / o10706 will not work.
Note 3: The primer combinations sul2/sul4 and sul7/sul9 should produce amplicons with all our sul-resistant lines.
Note 4: The primer CR3S is used as a right border primer only for lines, in which pGABI1 and pADIS1 have been used for transformation. It replaces o3144/35St in theses lines.