Genotyping and primer details


Genotyping and primer details for SALK_032917c - At3g02780
Line ID SALK_032917c
Locus At3g02780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:602443 of line SALK_032917c Chr3:602443
   
Insertion allele amplicon LBb3 - 33fr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 33fr: 5'-GTGGGTATCTACTGGCAAAACAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:603156
   
wt amplicon (over InsPos) 07hi - 33fr
wt amplicon start Chr3:602358
wt amplicon end Chr3:603156
wt amplicon size 799 bp
Locus specific primer 33fr: 5'-GTGGGTATCTACTGGCAAAACAAT-3'
2nd locus specific primer 07hi: 5'-TCGACTCTGTTTCCTTGTTATCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11