Genotyping and primer details


Genotyping and primer details for SALK_015524c - At4g21460
Line ID SALK_015524c
Locus At4g21460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:11430328 of line SALK_015524c Chr4:11430328
   
Insertion allele amplicon LBb3 - 33wj
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 33wj: 5'-ATCGAACGATGAAAATTAAAAACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:11430870
   
wt amplicon (over InsPos) 12wy - 33wj
wt amplicon start Chr4:11429726
wt amplicon end Chr4:11430870
wt amplicon size 1145 bp
Locus specific primer 33wj: 5'-ATCGAACGATGAAAATTAAAAACG-3'
2nd locus specific primer 12wy: 5'-AGTTTAATGAGCTCGGGGATATGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11