Genotyping and primer details


Genotyping and primer details for SALK_000573 - At2g38950
Line ID SALK_000573
Locus At2g38950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:16265151 of line SALK_000573 Chr2:16265151
   
Insertion allele amplicon LBb3 - 38us
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 38us: 5'-TGGCTTCTTAAATACTTCAGGGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:16266097
   
Insertion allele amplicon LBb3 - 51vr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 51vr: 5'-ATGGATGAGTTGAATCTTCTCGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:16264685
   
wt amplicon (over InsPos) 51vr - 38us
wt amplicon start Chr2:16264685
wt amplicon end Chr2:16266097
wt amplicon size 1413 bp
Locus specific primer 38us: 5'-TGGCTTCTTAAATACTTCAGGGAA-3'
2nd locus specific primer 51vr: 5'-ATGGATGAGTTGAATCTTCTCGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11