Genotyping and primer details


Genotyping and primer details for SALK_002556c - At2g22900
Line ID SALK_002556c
Locus At2g22900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9745367 of line SALK_002556c Chr2:9745367
   
Insertion allele amplicon LBb3 - 40us
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 40us: 5'-TACGGTTATGGTAACAGGATCACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9745822
   
wt amplicon (over InsPos) 33vx - 40us
wt amplicon start Chr2:9744762
wt amplicon end Chr2:9745822
wt amplicon size 1061 bp
Locus specific primer 40us: 5'-TACGGTTATGGTAACAGGATCACA-3'
2nd locus specific primer 33vx: 5'-TCTCCTTCGAGGTATATCTTTGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11