Genotyping and primer details


Genotyping and primer details for SALK_003155 - At3g26744
Line ID SALK_003155
Locus At3g26744 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:9833253 of line SALK_003155 Chr3:9833253
   
Insertion allele amplicon LBb3 - 62sa
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 62sa: 5'-TTGTTGTGTTGTAGCTTTGTAGGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:9833720
   
wt amplicon (over InsPos) 03sx - 62sa
wt amplicon start Chr3:9832624
wt amplicon end Chr3:9833720
wt amplicon size 1097 bp
Locus specific primer 62sa: 5'-TTGTTGTGTTGTAGCTTTGTAGGG-3'
2nd locus specific primer 03sx: 5'-TGCAAATGTTTTGTCTGTCTTCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11