Genotyping and primer details


Genotyping and primer details for SALK_003337c - At2g34720
Line ID SALK_003337c
Locus At2g34720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14651143 of line SALK_003337c Chr2:14651143
   
Insertion allele amplicon LBb3 - 68by
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 68by: 5'-TTAGGCCTCTGTTAGTCATTTCGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14651687
   
wt amplicon (over InsPos) 11da - 68by
wt amplicon start Chr2:14650519
wt amplicon end Chr2:14651687
wt amplicon size 1169 bp
Locus specific primer 68by: 5'-TTAGGCCTCTGTTAGTCATTTCGT-3'
2nd locus specific primer 11da: 5'-GACGTTGGGGCTGAGTAATAAATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11