Genotyping and primer details


Genotyping and primer details for SALK_007532c - At3g15780
Line ID SALK_007532c
Locus At3g15780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5342242 of line SALK_007532c Chr3:5342242
   
Insertion allele amplicon R204 - SC05
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer SC05: 5'-GACTTACTTGGTGCTCATGCAATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5342779
   
wt amplicon (over InsPos) 05cb - SC05
wt amplicon start Chr3:5341933
wt amplicon end Chr3:5342779
wt amplicon size 847 bp
Locus specific primer SC05: 5'-GACTTACTTGGTGCTCATGCAATA-3'
2nd locus specific primer 05cb: 5'-ATTTTGGCTTAGGCAATTAGGATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11