Genotyping and primer details


Genotyping and primer details for SALK_009112 - At1g44224
Line ID SALK_009112
Locus At1g44224 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:16821826 of line SALK_009112 Chr1:16821826
   
Insertion allele amplicon LBb3 - 18ux
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 18ux: 5'-TCTTTGAGGTCTTTGATCCATCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:16821249
   
wt amplicon (over InsPos) 54vl - 18ux
wt amplicon start Chr1:16821249
wt amplicon end Chr1:16822202
wt amplicon size 954 bp
Locus specific primer 18ux: 5'-TCTTTGAGGTCTTTGATCCATCTT-3'
2nd locus specific primer 54vl: 5'-TTAATTCCCCAAATCCAATTTACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11