Genotyping and primer details


Genotyping and primer details for SALK_009404c - At3g26910
Line ID SALK_009404c
Locus At3g26910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:9917327 of line SALK_009404c Chr3:9917327
   
Insertion allele amplicon LBb3 - 17jk
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 17jk: 5'-TCTATGATCAGCATTTACCTGTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:9916509
   
wt amplicon (over InsPos) 06ll - 17jk
wt amplicon start Chr3:9916509
wt amplicon end Chr3:9917817
wt amplicon size 1309 bp
Locus specific primer 17jk: 5'-TCTATGATCAGCATTTACCTGTGC-3'
2nd locus specific primer 06ll: 5'-TCATAACGACGAAGAGAGCAGTAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11