Genotyping and primer details


Genotyping and primer details for SALK_010040c - At4g13640
Line ID SALK_010040c
Locus At4g13640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:7936907 of line SALK_010040c Chr4:7936907
   
Insertion allele amplicon LBb3 - 10ii
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 10ii: 5'-CACAATCGTTACCACAGAGAAAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:7936187
   
wt amplicon (over InsPos) 06ir - 10ii
wt amplicon start Chr4:7936187
wt amplicon end Chr4:7937041
wt amplicon size 855 bp
Locus specific primer 10ii: 5'-CACAATCGTTACCACAGAGAAAAC-3'
2nd locus specific primer 06ir: 5'-AGCAATAGAGCACAAGAACAACTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11