Genotyping and primer details


Genotyping and primer details for SALK_010370c - At4g36830
Line ID SALK_010370c
Locus At4g36830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:17350077 of line SALK_010370c Chr4:17350077
   
Insertion allele amplicon LBb3 - 46us
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 46us: 5'-ATGTCAACGGCTCTGATTAACTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17349511
   
wt amplicon (over InsPos) 30vx - 46us
wt amplicon start Chr4:17349511
wt amplicon end Chr4:17350807
wt amplicon size 1297 bp
Locus specific primer 46us: 5'-ATGTCAACGGCTCTGATTAACTCT-3'
2nd locus specific primer 30vx: 5'-ACATTTTGGTGGGTATTGTGGTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11