Genotyping and primer details


Genotyping and primer details for SALK_010806 - At1g68590
Line ID SALK_010806
Locus At1g68590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:25758157 of line SALK_010806 Chr1:25758157
   
Insertion allele amplicon LBb3 - 32fx
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 32fx: 5'-TCAGCAAACCTGTGAAAATAAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:25758779
   
wt amplicon (over InsPos) 01hb - 32fx
wt amplicon start Chr1:25757682
wt amplicon end Chr1:25758779
wt amplicon size 1098 bp
Locus specific primer 32fx: 5'-TCAGCAAACCTGTGAAAATAAAGA-3'
2nd locus specific primer 01hb: 5'-AGATCCATGGTTTAGCTTCAAGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11