Genotyping and primer details


Genotyping and primer details for SALK_010926 - At1g01110
Line ID SALK_010926
Locus At1g01110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:54327 of line SALK_010926 Chr1:54327
   
Insertion allele amplicon LBb3 - 19ux
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 19ux: 5'-CTACTAGACCGTGGGATAAACGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:53794
   
wt amplicon (over InsPos) 53vl - 19ux
wt amplicon start Chr1:53794
wt amplicon end Chr1:54628
wt amplicon size 835 bp
Locus specific primer 19ux: 5'-CTACTAGACCGTGGGATAAACGAG-3'
2nd locus specific primer 53vl: 5'-ATCTTCCAAACACAACTCAACCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11