Genotyping and primer details


Genotyping and primer details for SALK_013388 - At2g30160
Line ID SALK_013388
Locus At2g30160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12878613 of line SALK_013388 Chr2:12878613
   
Insertion allele amplicon LBb3 - 24gm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 24gm: 5'-TCTCCCATGCTTTGTCTTTAGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12877832
   
wt amplicon (over InsPos) 01il - 24gm
wt amplicon start Chr2:12877832
wt amplicon end Chr2:12878721
wt amplicon size 890 bp
Locus specific primer 24gm: 5'-TCTCCCATGCTTTGTCTTTAGTTT-3'
2nd locus specific primer 01il: 5'-CATAAATCAACCAACCTTCCTCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11