Genotyping and primer details


Genotyping and primer details for SALK_014278c - At4g33770
Line ID SALK_014278c
Locus At4g33770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16193444 of line SALK_014278c Chr4:16193444
   
Insertion allele amplicon LBb3 - 12ty
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 12ty: 5'-AATTGTGAAAAAGCCAAAGTTGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16194100
   
wt amplicon (over InsPos) 23vb - 12ty
wt amplicon start Chr4:16193266
wt amplicon end Chr4:16194100
wt amplicon size 835 bp
Locus specific primer 12ty: 5'-AATTGTGAAAAAGCCAAAGTTGAT-3'
2nd locus specific primer 23vb: 5'-GGTGGGACGAGAGTTAGATAAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11