Genotyping and primer details

Genotyping and primer details for SALK_014446c - At3g07310
Line ID SALK_014446c
Locus At3g07310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2329675 of line SALK_014446c Chr3:2329675
Insertion allele amplicon LBb3 - 27mv
Locus specific primer 27mv: 5'-TCAGAGAGTTTAACACAGGCAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2328903
wt amplicon (over InsPos) 07oj - 27mv
wt amplicon start Chr3:2328903
wt amplicon end Chr3:2329839
wt amplicon size 937 bp
Locus specific primer 27mv: 5'-TCAGAGAGTTTAACACAGGCAAGA-3'
2nd locus specific primer 07oj: 5'-CTCTTCAACCTTATCCATTCTTCG-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11