Genotyping and primer details


Genotyping and primer details for SALK_015452c - At3g14870
Line ID SALK_015452c
Locus At3g14870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5005265 of line SALK_015452c Chr3:5005265
   
Insertion allele amplicon LBb3 - 34wu
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 34wu: 5'-AAGCTGGAGTGTCAGTTAAAGCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5004675
   
wt amplicon (over InsPos) 08xf - 34wu
wt amplicon start Chr3:5004675
wt amplicon end Chr3:5006030
wt amplicon size 1356 bp
Locus specific primer 34wu: 5'-AAGCTGGAGTGTCAGTTAAAGCTC-3'
2nd locus specific primer 08xf: 5'-AAGTCCATGATTCGAAATCACTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11