Genotyping and primer details


Genotyping and primer details for SALK_016116 - At2g16530
Line ID SALK_016116
Locus At2g16530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:7164413 of line SALK_016116 Chr2:7164413
   
Insertion allele amplicon R204 - 48we
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer 48we: 5'-TCCACATGATCAAAAGACAGAAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:7163768
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11