Genotyping and primer details


Genotyping and primer details for SALK_016439c - At4g21745
Line ID SALK_016439c
Locus At4g21745 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:11549659 of line SALK_016439c Chr4:11549659
   
Insertion allele amplicon LBb3 - 10va
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 10va: 5'-CCTACTGGTATAGGAGGCACATCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:11550258
   
wt amplicon (over InsPos) 05vj - 10va
wt amplicon start Chr4:11549318
wt amplicon end Chr4:11550258
wt amplicon size 941 bp
Locus specific primer 10va: 5'-CCTACTGGTATAGGAGGCACATCT-3'
2nd locus specific primer 05vj: 5'-TCTTCTCCATCAACATTCAACAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11