Genotyping and primer details


Genotyping and primer details for SALK_017597c - At2g32930
Line ID SALK_017597c
Locus At2g32930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:13968061 of line SALK_017597c Chr2:13968061
   
Insertion allele amplicon LBb3 - 17ht
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 17ht: 5'-ACGAGTTTTGATTTTGTGTCTGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13968467
   
wt amplicon (over InsPos) 09ix - 17ht
wt amplicon start Chr2:13967792
wt amplicon end Chr2:13968467
wt amplicon size 676 bp
Locus specific primer 17ht: 5'-ACGAGTTTTGATTTTGTGTCTGTG-3'
2nd locus specific primer 09ix: 5'-GTATCGACAAGAGTTTCCATAGCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11