Genotyping and primer details


Genotyping and primer details for SALK_017723 - At5g11930
Line ID SALK_017723
Locus At5g11930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3845490 of line SALK_017723 Chr5:3845490
   
Insertion allele amplicon R204 - CG33
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer CG33: 5'-CTTACCGACTGGCAAAAGATAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3845003
   
Insertion allele amplicon R212 - CG34
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer CG34: 5'-GATGCCCATAACCATTATATGTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3845932
   
wt amplicon (over InsPos) CG34 - CG33
wt amplicon start Chr5:3845003
wt amplicon end Chr5:3845932
wt amplicon size 930 bp
Locus specific primer CG33: 5'-CTTACCGACTGGCAAAAGATAAAG-3'
2nd locus specific primer CG34: 5'-GATGCCCATAACCATTATATGTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11