Genotyping and primer details


Genotyping and primer details for SALK_017956 - At1g35470
Line ID SALK_017956
Locus At1g35470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:13053991 of line SALK_017956 Chr1:13053991
   
Insertion allele amplicon LBb3 - 23ih
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 23ih: 5'-TGAGGTTTGATTACACAGGAACAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:13053362
   
wt amplicon (over InsPos) 06kj - 23ih
wt amplicon start Chr1:13053362
wt amplicon end Chr1:13054495
wt amplicon size 1134 bp
Locus specific primer 23ih: 5'-TGAGGTTTGATTACACAGGAACAG-3'
2nd locus specific primer 06kj: 5'-TCTTTGTTGATCATTGTGTGTTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11