Genotyping and primer details


Genotyping and primer details for SALK_020806c - At2g03450
Line ID SALK_020806c
Locus At2g03450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:1043328 of line SALK_020806c Chr2:1043328
   
Insertion allele amplicon LBb3 - 49xh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 49xh: 5'-ATGTGGTGCCTGTAGGTCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:1044186
   
Insertion allele amplicon R204 - BI36
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer BI36: 5'-AGGTTGGGAGTGATTTAAAAGGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:1042302
   
wt amplicon (over InsPos) BI37 - BI36
wt amplicon start Chr2:1042302
wt amplicon end Chr2:1043438
wt amplicon size 1137 bp
Locus specific primer BI36: 5'-AGGTTGGGAGTGATTTAAAAGGAT-3'
2nd locus specific primer BI37: 5'-CAATGTACCACAAGACTGCAAAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11