Genotyping and primer details


Genotyping and primer details for SALK_020960c - At4g18640
Line ID SALK_020960c
Locus At4g18640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:10261734 of line SALK_020960c Chr4:10261734
   
Insertion allele amplicon R204 - 03as
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer 03as: 5'-TTGGTACCCCTACATTTTGTTCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10262221
   
wt amplicon (over InsPos) 02cb - 03as
wt amplicon start Chr4:10261414
wt amplicon end Chr4:10262221
wt amplicon size 808 bp
Locus specific primer 03as: 5'-TTGGTACCCCTACATTTTGTTCTC-3'
2nd locus specific primer 02cb: 5'-GAAAACTTGGACACTGGTAAGCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11