Genotyping and primer details


Genotyping and primer details for SALK_021618c - At2g47490
Line ID SALK_021618c
Locus At2g47490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:19489284 of line SALK_021618c Chr2:19489284
   
Insertion allele amplicon LBb3 - 25mh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 25mh: 5'-TTTCCCACATATGAGATGATCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19488762
   
wt amplicon (over InsPos) 13oj - 25mh
wt amplicon start Chr2:19488762
wt amplicon end Chr2:19489688
wt amplicon size 927 bp
Locus specific primer 25mh: 5'-TTTCCCACATATGAGATGATCAAA-3'
2nd locus specific primer 13oj: 5'-AATATCATGGATCCAAAGCTCATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11