Genotyping and primer details


Genotyping and primer details for SALK_021965c - At2g41070
Line ID SALK_021965c
Locus At2g41070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17131506 of line SALK_021965c Chr2:17131506
   
Insertion allele amplicon LBb3 - 66sa
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 66sa: 5'-TTTGTTCCCTTATTCACAATCTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17131053
   
wt amplicon (over InsPos) 11sx - 66sa
wt amplicon start Chr2:17131053
wt amplicon end Chr2:17131845
wt amplicon size 793 bp
Locus specific primer 66sa: 5'-TTTGTTCCCTTATTCACAATCTCC-3'
2nd locus specific primer 11sx: 5'-CTTGATCATCCTCTTCTGTCTCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11