Genotyping and primer details


Genotyping and primer details for SALK_024277c - At1g18460
Line ID SALK_024277c
Locus At1g18460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6354683 of line SALK_024277c Chr1:6354683
   
Insertion allele amplicon LBb3 - 24ux
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 24ux: 5'-CTTCGATTTTACGGGTGATTACAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6355237
   
wt amplicon (over InsPos) 34vw - 24ux
wt amplicon start Chr1:6354020
wt amplicon end Chr1:6355237
wt amplicon size 1218 bp
Locus specific primer 24ux: 5'-CTTCGATTTTACGGGTGATTACAT-3'
2nd locus specific primer 34vw: 5'-ATGTGTTCTACTTTGGCAGGATCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11