Genotyping and primer details


Genotyping and primer details for SALK_025224c - At1g17480
Line ID SALK_025224c
Locus At1g17480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6007122 of line SALK_025224c Chr1:6007122
   
Insertion allele amplicon LBb3 - 13rb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 13rb: 5'-TCTCGTGTAAGAGCTCATCGTAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6007497
   
wt amplicon (over InsPos) 20rz - 13rb
wt amplicon start Chr1:6006633
wt amplicon end Chr1:6007497
wt amplicon size 865 bp
Locus specific primer 13rb: 5'-TCTCGTGTAAGAGCTCATCGTAGA-3'
2nd locus specific primer 20rz: 5'-CTGTTGAATATGGCTTGACAAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11