Genotyping and primer details


Genotyping and primer details for SALK_025426c - At2g40290
Line ID SALK_025426c
Locus At2g40290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:16829276 of line SALK_025426c Chr2:16829276
   
Insertion allele amplicon LBb3 - 14ss
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 14ss: 5'-ATTTCATTTGATTTTCTCACCCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:16829872
   
wt amplicon (over InsPos) 14ue - 14ss
wt amplicon start Chr2:16828954
wt amplicon end Chr2:16829872
wt amplicon size 919 bp
Locus specific primer 14ss: 5'-ATTTCATTTGATTTTCTCACCCAT-3'
2nd locus specific primer 14ue: 5'-CCTTGTCAAAGCTTAAACCAAAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11