Genotyping and primer details


Genotyping and primer details for SALK_026362c - At2g25480
Line ID SALK_026362c
Locus At2g25480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10844995 of line SALK_026362c Chr2:10844995
   
Insertion allele amplicon LBb3 - 38wm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 38wm: 5'-TGTCATGTGATGCAGTTCTATTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10844510
   
wt amplicon (over InsPos) 26xa - 38wm
wt amplicon start Chr2:10844510
wt amplicon end Chr2:10845394
wt amplicon size 885 bp
Locus specific primer 38wm: 5'-TGTCATGTGATGCAGTTCTATTCA-3'
2nd locus specific primer 26xa: 5'-TTACTGTTCCTTTTTCGCTCCTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11