Genotyping and primer details


Genotyping and primer details for SALK_027039c - At3g17240
Line ID SALK_027039c
Locus At3g17240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5891059 of line SALK_027039c Chr3:5891059
   
Insertion allele amplicon LBb3 - 20md
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 20md: 5'-CAACTTCAGGGTACGTGTAGACAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5890607
   
wt amplicon (over InsPos) 03ng - 20md
wt amplicon start Chr3:5890607
wt amplicon end Chr3:5891533
wt amplicon size 927 bp
Locus specific primer 20md: 5'-CAACTTCAGGGTACGTGTAGACAA-3'
2nd locus specific primer 03ng: 5'-ATTAGGCCCTTCTTCACTCTTCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11