Genotyping and primer details


Genotyping and primer details for SALK_028545 - At2g26790
Line ID SALK_028545
Locus At2g26790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11427721 of line SALK_028545 Chr2:11427721
   
Insertion allele amplicon R204 - AW63
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer AW63: 5'-CATCACCAGCCAGATATTTCCTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11426829
   
wt amplicon (over InsPos) AW64 - AW63
wt amplicon start Chr2:11426829
wt amplicon end Chr2:11427770
wt amplicon size 942 bp
Locus specific primer AW63: 5'-CATCACCAGCCAGATATTTCCTAT-3'
2nd locus specific primer AW64: 5'-ATTTAAATGGATCGGAGTGTTCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11