Genotyping and primer details


Genotyping and primer details for SALK_028863 - At4g31080
Line ID SALK_028863
Locus At4g31080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15122247 of line SALK_028863 Chr4:15122247
   
Insertion allele amplicon LBb3 - 11vt
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 11vt: 5'-AAAAATGGTTGTATAGTTGACCGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15121783
   
wt amplicon (over InsPos) 71wi - 11vt
wt amplicon start Chr4:15121783
wt amplicon end Chr4:15122824
wt amplicon size 1042 bp
Locus specific primer 11vt: 5'-AAAAATGGTTGTATAGTTGACCGC-3'
2nd locus specific primer 71wi: 5'-CAAGTCCTGCATTTACAAAGCATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11