Genotyping and primer details


Genotyping and primer details for SALK_029299 - At1g66340
Line ID SALK_029299
Locus At1g66340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:24737628 of line SALK_029299 Chr1:24737628
   
Insertion allele amplicon LBb3 - 41cp
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 41cp: 5'-CATCCATAACTCGATCCAAATTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:24737683
   
wt amplicon (over InsPos) 27dk - 41cp
wt amplicon start Chr1:24736684
wt amplicon end Chr1:24737683
wt amplicon size 1000 bp
Locus specific primer 41cp: 5'-CATCCATAACTCGATCCAAATTCT-3'
2nd locus specific primer 27dk: 5'-GTGAATCTGATGGAGGGTAACATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11