Genotyping and primer details


Genotyping and primer details for SALK_029432c - F24K9
Line ID SALK_029432c
Locus F24K9 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3580589 of line SALK_029432c Chr3:3580589
   
Insertion allele amplicon LBb3 - 36vi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 36vi: 5'-ACAAGAAAGGAGATCACTGGACAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3579909
   
wt amplicon (over InsPos) 66vw - 36vi
wt amplicon start Chr3:3579909
wt amplicon end Chr3:3581015
wt amplicon size 1107 bp
Locus specific primer 36vi: 5'-ACAAGAAAGGAGATCACTGGACAT-3'
2nd locus specific primer 66vw: 5'-CATTGTCCGATGATGAATTAGATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11